Research Reports

Preliminary Laboratory Investigation of Enzyme Solutions as a Soil Stabilizer

Principal Investigator:

Mihai O Marasteanu, Ray Hozalski, Timothy R Clyne, Raul Velasquez

June 2005

Report no. Mn/DOT 2005-25

Projects: Preliminary Laboratory Investigation of a Commercial Enzyme Solution as a Soil Stabilizer

Topics: Pavement design for cold climates

This research studied the effect of two enzymes as soil stabilizers on two soil types to determine how and under what conditions they function. Researchers evaluated the chemical composition, mode of action, resilient modulus, and shear strength to determine the effects of the enzymes A and B on the soils I and II. The enzymes produced a high concentration of protein and observations suggest the enzymes behave like a surfactant, which effects its stabilization performance. The specimens were subjected to testing of varying lengths of time to determine their performance. Researchers observed an increase in the resilient modulus as the curing time increased but that an increase in application rate, as suggested by manufacturers, did not improve the performance of the enzymes. The study also suggests noticeable differences between the two enzymes and their effects on the soils in terms of resilient modulus and the stiffness of the soil.

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